My vlog is related to molecular genetics and genetics engineering.Lets start a blog.Discoveries in microbial genetics have provided the basics for the discipline of molecular genetics, which is concerned with the analysis and manipulation of DNA using biochemical and micro biological techniques.It has been stated that these techniques have revolutionized the study of biology and medicine, probably more than any technique since the development of the light microscope.Some techniques and application of molecular genetics are discussed below.

GENETIC ENGINEERING:The Most important application of molecular genetics in biotechnology is genetic engineering or recombinant DNA technology.This consist of isolation of the genes coding for any desired protein from micro organisms or from cells of higher forms of life including human beings, and their introduction into suitable micro organisms,in which the genes would be functional,directing the production of the specific protein.Such cloning of genes in micro organisms enables the preparation of the desired protein in pure form,in large quantities and at a reasonable cost.

Different strategies have been employed for obtaining the desired genes.For very smaal proteins,such as the pituitary hormone somatostatin whose complete amino acid sequence are known,the genes can be synthesized in the laboratory.With large protein, this is not possible.The DNA can be cleaved by specific enzyme called restrictions endonucleases and the fragments containing the desired genes isolated.This does not work with DNA of higher organism as they contain introns.In such cases, the messenger RNA concerned can be isolated from cells producing the desired protein.A DNA copy is made from the mRNA using the enzyme reverse transriptase.The double stranded DNA gene is then prepared using DNA polymerase.This is incorporated into suitable vector or carriers,such as plasmids or temperate bacteriophage, for insertion into micro organisms.The micro organisms commonly employed is Eschershia coli, though many other bacteria and yeast have also been used.

Genetics engineering has become an established branch of biotechnology with great scope for commercial exploitation.Cloned human insulin,interferons somatostatin, growth hormone and many other biological have already been marketed.Safer vaccine can be produced by cloning the protective antigen of pathogens,as has already been done,as in the case of foot and mouth disease, and hepatitis B and rabies viruses.This versatile techniques has many extra Medical application .Thank you for your listening and reading.Thank you.