My vlog is Intended uses dengue

The pabio dengue igm capture Elisa is for the qualitative detection of igm dengue antibodies to dengue antigen in serum as an aid in the clinical laboratory diagnosis of patient with clinical symptoms consistent with dengue fever. The dengue igm capture Elisa should be used in conjunction with other dengue serology. 

Introduction 

Dengue a flavor virus is found in large area of the tropics and subtropics. Transmission Loop 9 by mosquitoes,aedes aegypti and added albopictus. Dengue virus infection cause a spectrum of clinical manifestation ranging from un apparent to fatal hemorrhagic disease. Classic dengue or break bone fever is characterized by the sudden onset of fever intense headache myalgia arthralgia and rash.A diphasic febrile course is common as is insomnia and anorexia with loss of taste or bitter taste. Dengue haemorrhagic fever and dengue shock syndrome are severe complications often associated with a second serotype infection

Detection of igm antibodies to dengue virus by Elisa is a valuable procedure particularly in second and subsequently infection where the occurrence of complications is high serum igm antibodies can be detected from dengue patient as early as three to five days after the onset of fever and generally persist for 30 to 90 day although detectable levels may be present eight months post infection

Material provided

Antibodies coated micro well are coated with anti human igm antibodies ready for use unused micro well should be resealed immediately and Store in the presence of the desiccant stable at 2 to 8 degree celcius until expiry dengue 1 to 4 antigen one clear capped vial 150 ul blue concentrated dengue viral antigen 1,2,3 and 4 with preservative unused diluted antigens must be discarded

Four types of Elisa 

Plate Coating

Plate Blocking

Antibody Incubation Detection. The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples.

An ELISA test uses specialized enzymes that attach to antibodies in your blood. The test involves mixing a sample of your blood with a known compound on special absorbent plates. A color change to blue indicates the presence of the antigen-antibody complex. The more antibody bound to antigen

 The antigen is immobilized to the well of a plate by adsorption, or captured with a bound, antigen-specific antibody. A detection antibody is then added forming a complex with the antigen, if present.thank you to all